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anti human ace2 phycoerythrin pe  (Bioss)


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    Bioss anti human ace2 phycoerythrin pe
    SARS-CoV-2 infection induces MC accumulation around cerebrovascular region in mice. BALB/c mice were infected intranasally with SARS-CoV-2 501Y.V2 strain at the dose of 7 × 10 4 CCID 50 . The brains were collected at 3 dpi. Brain injury was observed by H.E. staining of brain sections (A, B) and T. blue staining was used to observe MCs (E, F) . Scale bar: 50 µm. (C) <t>ACE2</t> expression in hCMEC/D3 and HMC3 detected by flow cytometry with immunostaining with specific antibody. (D) Viral infection. hCMEC/D3 and HMC3 cells were infected with SARS-CoV-2 spike-pseudotyped lentivirus (HIV-NL4-3/spike) (5 ng p24 gag ) for 48h, and viral infection was determined by measuring the luciferase activity. One representative result from three independent repeats is shown. Data are presented as M ± SD . *** p < 0.001 is considered significant differences.
    Anti Human Ace2 Phycoerythrin Pe, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human ace2 phycoerythrin pe/product/Bioss
    Average 94 stars, based on 31 article reviews
    anti human ace2 phycoerythrin pe - by Bioz Stars, 2026-02
    94/100 stars

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    1) Product Images from "Mast cell activation triggered by SARS-CoV-2 causes inflammation in brain microvascular endothelial cells and microglia"

    Article Title: Mast cell activation triggered by SARS-CoV-2 causes inflammation in brain microvascular endothelial cells and microglia

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2024.1358873

    SARS-CoV-2 infection induces MC accumulation around cerebrovascular region in mice. BALB/c mice were infected intranasally with SARS-CoV-2 501Y.V2 strain at the dose of 7 × 10 4 CCID 50 . The brains were collected at 3 dpi. Brain injury was observed by H.E. staining of brain sections (A, B) and T. blue staining was used to observe MCs (E, F) . Scale bar: 50 µm. (C) ACE2 expression in hCMEC/D3 and HMC3 detected by flow cytometry with immunostaining with specific antibody. (D) Viral infection. hCMEC/D3 and HMC3 cells were infected with SARS-CoV-2 spike-pseudotyped lentivirus (HIV-NL4-3/spike) (5 ng p24 gag ) for 48h, and viral infection was determined by measuring the luciferase activity. One representative result from three independent repeats is shown. Data are presented as M ± SD . *** p < 0.001 is considered significant differences.
    Figure Legend Snippet: SARS-CoV-2 infection induces MC accumulation around cerebrovascular region in mice. BALB/c mice were infected intranasally with SARS-CoV-2 501Y.V2 strain at the dose of 7 × 10 4 CCID 50 . The brains were collected at 3 dpi. Brain injury was observed by H.E. staining of brain sections (A, B) and T. blue staining was used to observe MCs (E, F) . Scale bar: 50 µm. (C) ACE2 expression in hCMEC/D3 and HMC3 detected by flow cytometry with immunostaining with specific antibody. (D) Viral infection. hCMEC/D3 and HMC3 cells were infected with SARS-CoV-2 spike-pseudotyped lentivirus (HIV-NL4-3/spike) (5 ng p24 gag ) for 48h, and viral infection was determined by measuring the luciferase activity. One representative result from three independent repeats is shown. Data are presented as M ± SD . *** p < 0.001 is considered significant differences.

    Techniques Used: Infection, Staining, Expressing, Flow Cytometry, Immunostaining, Luciferase, Activity Assay



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    Adhesive diabetic MPs facilitate the entry of SARS-CoV-2. SARS-CoV-2 enters the host cells via binding of its spike (S) protein to the <t>ACE2</t> receptor expressed on the cell membrane. ACE2 expressed on microparticles (MPs) enables the MPs to bind SARS-CoV-2. When compared with normal MPs ( left ), diabetic MPs ( right ) have larger externalized phosphatidylserine surface and expressed more tissue factors, which leads to the high adhesiveness and procoagulant activity of diabetic MPs. Excessively increased diabetic MPs carry the SARS-CoV-2 into the cells through PS-mediated adhesion and endocytosis and hence provide additional doorway for virus entry. MPs, microparticles; PS, phosphatidylserine. Graph was created with BioRender.com and published with permission.
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    SARS-CoV-2 infection induces MC accumulation around cerebrovascular region in mice. BALB/c mice were infected intranasally with SARS-CoV-2 501Y.V2 strain at the dose of 7 × 10 4 CCID 50 . The brains were collected at 3 dpi. Brain injury was observed by H.E. staining of brain sections (A, B) and T. blue staining was used to observe MCs (E, F) . Scale bar: 50 µm. (C) ACE2 expression in hCMEC/D3 and HMC3 detected by flow cytometry with immunostaining with specific antibody. (D) Viral infection. hCMEC/D3 and HMC3 cells were infected with SARS-CoV-2 spike-pseudotyped lentivirus (HIV-NL4-3/spike) (5 ng p24 gag ) for 48h, and viral infection was determined by measuring the luciferase activity. One representative result from three independent repeats is shown. Data are presented as M ± SD . *** p < 0.001 is considered significant differences.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Mast cell activation triggered by SARS-CoV-2 causes inflammation in brain microvascular endothelial cells and microglia

    doi: 10.3389/fcimb.2024.1358873

    Figure Lengend Snippet: SARS-CoV-2 infection induces MC accumulation around cerebrovascular region in mice. BALB/c mice were infected intranasally with SARS-CoV-2 501Y.V2 strain at the dose of 7 × 10 4 CCID 50 . The brains were collected at 3 dpi. Brain injury was observed by H.E. staining of brain sections (A, B) and T. blue staining was used to observe MCs (E, F) . Scale bar: 50 µm. (C) ACE2 expression in hCMEC/D3 and HMC3 detected by flow cytometry with immunostaining with specific antibody. (D) Viral infection. hCMEC/D3 and HMC3 cells were infected with SARS-CoV-2 spike-pseudotyped lentivirus (HIV-NL4-3/spike) (5 ng p24 gag ) for 48h, and viral infection was determined by measuring the luciferase activity. One representative result from three independent repeats is shown. Data are presented as M ± SD . *** p < 0.001 is considered significant differences.

    Article Snippet: For detecting the expression of HLA-DR and ACE2, cells were incubated with anti-human HLA-DR allophycocyanin (APC) (Tonbo Biosciences, San Diego, USA, 20-9952) or anti-human ACE2-phycoerythrin (PE) (Bioss, Beijing, China, bs-1004R) at 4°C for 30 min and detected with flow cytometry (BD Accuri C6).

    Techniques: Infection, Staining, Expressing, Flow Cytometry, Immunostaining, Luciferase, Activity Assay

    Adhesive diabetic MPs facilitate the entry of SARS-CoV-2. SARS-CoV-2 enters the host cells via binding of its spike (S) protein to the ACE2 receptor expressed on the cell membrane. ACE2 expressed on microparticles (MPs) enables the MPs to bind SARS-CoV-2. When compared with normal MPs ( left ), diabetic MPs ( right ) have larger externalized phosphatidylserine surface and expressed more tissue factors, which leads to the high adhesiveness and procoagulant activity of diabetic MPs. Excessively increased diabetic MPs carry the SARS-CoV-2 into the cells through PS-mediated adhesion and endocytosis and hence provide additional doorway for virus entry. MPs, microparticles; PS, phosphatidylserine. Graph was created with BioRender.com and published with permission.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Increased circulating microparticles contribute to severe infection and adverse outcomes of COVID-19 in patients with diabetes

    doi: 10.1152/ajpheart.00409.2022

    Figure Lengend Snippet: Adhesive diabetic MPs facilitate the entry of SARS-CoV-2. SARS-CoV-2 enters the host cells via binding of its spike (S) protein to the ACE2 receptor expressed on the cell membrane. ACE2 expressed on microparticles (MPs) enables the MPs to bind SARS-CoV-2. When compared with normal MPs ( left ), diabetic MPs ( right ) have larger externalized phosphatidylserine surface and expressed more tissue factors, which leads to the high adhesiveness and procoagulant activity of diabetic MPs. Excessively increased diabetic MPs carry the SARS-CoV-2 into the cells through PS-mediated adhesion and endocytosis and hence provide additional doorway for virus entry. MPs, microparticles; PS, phosphatidylserine. Graph was created with BioRender.com and published with permission.

    Article Snippet: APC Mouse Anti-Human CD45 (Cat. No. 555485, BD Biosciences), PE Mouse Anti-Human CD144 (Cat. No. 560410, BD Biosciences), BV421 Mouse Anti-Human CD61 (Cat. No. 744381, BD Biosciences), APC Anti-Human CD142 (Cat. No. 130-098-745, Milteny Biotec), and PE Anti-ACE2 (Cat. No. bs-1004r-pe, Bioss) antibodies were used for MP labeling.

    Techniques: Binding Assay, Activity Assay